Jasem, can you post instructions on how to perform that update on the Pi3? I installed the latest indi-asi update today, but the USB2 ASI120MM still won't capture any images, although the camera is recognized by Ekos. However, when I try to capture an image, the timer either counts down to 0.01" and then stops, freezing Ekos to the point where I cannot disconnect the camera or stop Indi, I need to force quit Ekos and restart it, or it will loop endlessly without ever rendering a frame.
I am running all services directly on the mount, nothing remotely on my desktop, I merely connect to the Pi3 via VNC.

I set the Proportional Gain as you indicated but to no avail as you can see in the screenshot. I'm not sure if my guide scope is focused well enough though. I ordered a new one with a better focuser so maybe that'll help.

Nevermind, I figured it out.
indilib.org/devices/ccds/zwo-optics-asi-cameras.html
I had flashed the firmware, but I had a) not selected to OVERWRITE the old firmware and b) used the ASI120MM.icc file, not the ASI120MM-compatible.icc file to change the firmware.
Now that I flashed the firmware with the -compatible version, the USB2 version of the ASI120MM captures and loops images just fine.
That does not resolve the original problem for which I opened this topic, though, i.e. that ST4 guiding through the ASI120MM-S (USB3 version) does not work.

Give PHD2 or LinGuider a go and see if the results are different.

Did you try sending pulses manually via the INDI Control Panel like you did with the USB2 version?

Yes, that was part of the original troubleshooting. I have not tried it with the USB2 version yet since the firmware flash, but since it worked before and since it works for you, I assume that will not change. The ST4 guiding through the USB3 version is the problem. wvreeven (see a few comments above) found exactly the same problem, during calibration the mount never reverses, but drifts away into one direction, so it can't be my camera or isolated to the settings on my Pi3, as the issue reproduces on his setup.

It’s probably worth noting that my setup doesn’t use an RPi but simply a (2010) Dell laptop with Ubuntu Mate 17.10.1 (artful).

If USB3 version produces same problem, it's probably time to call ZWO about this.

And tell or ask them what exactly? Remember, guidng via ST4 on the USB3 versions works in PHD2. Just not in Ekos...

In PHD2 you're using ZWO driver or INDI driver?

I use the ZWO driver and on-cam guiding. I didn't even know it was possible to use the INDI driver in PHD2.

I was using the ZWO driver that shows up in the PhD2 drop down menu. I don't remember whether that was there before I installed Indi and Ekos and whether the PhD2 ZWO driver is dependent upon the installation of Indi or whether the driver was included in one of the last PhD2 updates.
I agree with wvreeven: This problem's got to be rooted somewhere in Ekos, since the ST4 guiding worked until the January 29 update. Besides, nobody working at ZWO, they are out of the office until February 23 to celebrate Chinese New Year.