What type of scope are you using SCT, refractor, etc?
Any idea of approx AZ of M81 when image was taken?
Do you have any flex between guide and main scope?
I took your image and did a plate solve on it, shows about 30arcsec of 'tadpoles', see attached.
This looks like a 'jump' happened.
When image starts, has slew and guider been stable for a number of seconds prior to image taking?